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Neoblast Markers piwi & vasa Are Expressed During Regeneration and Reproduction in Aeolosoma viride
Author(s) -
Hsieh Yu-Wen,
Chu Chia-Ying,
Chen Jiun-Hong
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.905.22
Subject(s) - planarian , regeneration (biology) , biology , lernaean hydra , annelid , microbiology and biotechnology , somatic cell , stem cell , asexual reproduction , blastema , anatomy , gene , genetics
Some animals can regenerate their lost tissues or organs. Both Tissue remodeling and organ reformation need cell proliferation and/or differentiation during regeneration. Neoblasts, one of the somatic stem cells, can proliferates to increase cell number in invertebrates, such as hydra and planarian. Though hydra and planarian are good model animals for regeneration research, their body plans are too simple to monitor regeneration of highly specific organs. Aeolosoma viride, a fresh water annelid with 19 segments, central nerve system, and a complete digestive system, present great regeneration ability. To trace neoblast, Avpiwi1, Avpiwi2, and Avvasa have been partly cloned. The sequences were used as probes to study A. viride neoblasts. These genes were found to be highly expressed in posterior growth region. Furthermore, Avpiwi1 was also expressed in the regenerating tissue. The expression level of these genes decreased and the regeneration process was blocked after 90 Gy r‐irradiation. These data strongly suggested that Avpiwi1, Avpiwi2, and Avvasa can label the neoblasts, which participate in A. viride regeneration. It means that the function of somatic stem cell may also be conserved in annelids.