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Evidence that glial cells modulate synaptic transmission in the NTS neurons sending projections to RVLM
Author(s) -
Accorsi-Mendonça Daniela,
Bonagamba Leni GH,
Machado Benedito H
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.903.3
Subject(s) - ppads , rostral ventrolateral medulla , neurotransmission , bursting , excitatory postsynaptic potential , chemistry , glutamatergic , glutamate receptor , neuroscience , brainstem , antagonist , purinergic receptor , patch clamp , receptor , central nervous system , biology , biochemistry , medulla oblongata
The peripheral chemoreflex activation produces autonomic and respiratory responses. In previous study we verified that ATP modulates the glutamatergic transmission onto NTS neurons sending projections to RVLM, which are related to the sympathoexcitatory component of chemoreflex. Here, we evaluated the role of glial cells in ATP release and the purinergic subtype receptors involved with this modulation. Adult rats received fluorescent tracer microinjections into RVLM and 7 days later the labeled NTS neurons sending projections to RVLM were identified in brainstem slices. TS evoked excitatory pos‐synaptic currents (TS‐eEPSCs) were recorded using patch clamp. Inhibition of glial cells with fluoracetate (FAC− 1mM) decreased the amplitude of TS‐eEPSCs and this effect was independent of glutamine in the bath (FAC: − 56 ± 0.06%, n=6; FAC + glutamine: −65 ± 0.11%, n=10). iso‐PPADS (100 μM), a P2X receptor antagonist, or A317491 (10 μM), a P2X 3 or P2X 2/3 receptor antagonist, produced similar decrease in the amplitude of TS‐eEPSCs (iso‐PPADS: −58 ± 7%, n=6; A317491: −57± 5%, n=5%). The subsequent addition of iso‐PPADS in the bath during FAC perfusion did not alter the glial inhibition effect on TS‐eEPSCs (FAC: −58 ± 11%; iso‐PPADS + FAC: −45 ± 6%, n=5). We conclude that glial cells modulate the synaptic transmission in the NTS neurons sending projections to RVLM. Supported by FAPESP and CNPq.