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Real‐Time Dynamics of Translation
Author(s) -
Puglisi Joseph Daniel,
Chen Jin,
Kornberg Guy,
O'Leary Sean,
Petrov Alexey,
Tsai Albert
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.90.1
Subject(s) - translation (biology) , ribosome , translational regulation , computational biology , translational frameshift , dynamics (music) , protein biosynthesis , transfer rna , ribosomal rna , eukaryotic translation , internal ribosome entry site , biology , messenger rna , microbiology and biotechnology , rna , genetics , gene , physics , acoustics
Translation of proteins by the ribosome represents the endpoint of gene expression and is highly regulated. The structures of ribosomal complexes represent a triumph of structural biology. Yet these structures lack a time axis: dynamic measurements are needed to understand the mysteries of translation. Here we present our broad efforts to develop and use single‐molecule fluorescence methods to track translation in real time. Using fluorescent labels on transfer RNAs, factors, mRNAs and ribosomal subunits, we have mapped conformational and compositional dynamics of the translational machinery during protein synthesis. Our focus has recently been on the dynamics of initiation, by which translational reading frame is established.