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Pattern of breathing in a mouse model of Down Syndrome (DS)
Author(s) -
DeRuisseau Lara Roberts,
Murphy Stacy L,
Zabycz Sylvia A,
DeRuisseau Keith C
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.899.4
Subject(s) - hypercapnia , tidal volume , ventilation (architecture) , plethysmograph , medicine , respiratory minute volume , cardiorespiratory fitness , respiratory system , respiration , room air distribution , respiratory rate , anesthesia , respiratory frequency , control of respiration , cardiology , heart rate , anatomy , blood pressure , mechanical engineering , physics , engineering , thermodynamics
DS results from trisomy 21 and is the most common cause of mental retardation in the US. Additionally, people with DS display muscle weakness and cardiorespiratory complications that may contribute to their altered breathing. We hypothesized that Ts65Dn mice, a model of DS, would exhibit an altered pattern of breathing compared to control mice. Unrestrained barometric plethysmography was used to quantify frequency (F; breaths/min), tidal volume (TV; mL/breath), and minute ventilation (MV; mL/min) in 12 month colony control (n=6) and Ts65Dn (n=6) mice during exposure to room air and hypercapnia (7% CO 2 , balanced air). During quiet breathing, Ts65Dn mice displayed reductions (control vs. Ts65Dn; mean ± sem; p<0.05) in TV (0.36 ± 0.02 vs. 0.26 ± 0.02) and MV (60.0 ± 6.1 vs. 40.0 ± 2.9), with no differences in F (168 ± 9 vs. 152 ± 7). When MV with room air was normalized to expired CO 2 the differences between groups remained. The breathing response to hypercapnia was elevated compared to baseline in both groups, with differences between groups in F (371 ± 19 vs. 315 ± 15 bpm), TV (0.62 ± 0.11 vs. 0.42 ± 0.04 mL/breath), and MV (188.2 ± 9.3 vs. 135.8 ± 18 mL/min). These preliminary findings indicate the Ts65Dn mouse has an altered pattern of breathing compared to control mice. These data also suggest that Ts65Dn mice are an appropriate model to study ventilation in DS, although further investigation is warranted. LMC R&D Committee.