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Functional expression of calcium sensing receptor in rat vagal pulmonary sensory neurons
Author(s) -
Gu Qihai,
Kagira Martin,
Gilbert Carolyn
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.894.18
Subject(s) - calcium sensing receptor , sensory system , receptor , extracellular , calcium imaging , medicine , endocrinology , calcium , calcium metabolism , neuroscience , calcium in biology , sensory neuron , receptor expression , homeostasis , sensory nerve , biology , microbiology and biotechnology
The calcium sensing receptor (CaSR), first isolated from bovine and human parathyroid, is a G‐protein‐coupled receptor that monitors the systemic, extracellular, free ionized‐calcium level in organs involved in systemic Ca 2+ homeostasis. Recent studies have shown that CaSR is also expressed in many tissues that are not directly related to the plasma Ca 2+ regulation, such as central and peripheral nervous systems where the function of this receptor remains to be defined. In this study, we examined the functional expression of CaSR in rat vagal pulmonary sensory neurons. Our immunohistochemical experiments demonstrated the expression of CaSR in these sensory neurons, as well as in the trachea and lungs. Whole‐cell patch‐clamp recordings were performed in isolated pulmonary sensory neurons. The results showed that pretreatment with NPS 2390 or NPS 2143, a selective CaSR antagonist, significantly enhanced the capsaicin‐evoked whole‐cell inward current. These data suggest that CaSR is likely involved in the integration of primary pulmonary sensory inputs under physiologic and/or pathophysiologic conditions. Funded partially by AHA 0835320N and MUSM startup funds.