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Carotid Body Artery Removal (CBX) Prevents Training‐ Induced Plasticity in Preautonomic Oxytocinergic Neurons of the Paraventricular Nucleus SHR
Author(s) -
Cruz Josiane Campos,
Cavalleri Marina Tuppy,
Michelini Lisete Compagno
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.891.4
Subject(s) - oxytocin , endocrinology , medicine , tonic (physiology) , chemistry
We observed that sinoaortic denervation prevents T‐induced plasticity in the OTergic preautonomic PVN neurons of SHR (Cavalleri et al., AJP, Regulatory, 2011). In the present study, we sought to investigate whether peripheral chemoreceptors participate in T‐induced OTergic plasticity in PVN subnuclei of SHR. CBX or SHAM were trained or kept sedentary (S) for 3 month. After cardiovascular records, rats were anesthetized and perfused (saline or PFA 4%). Tissue samples of PVN were processed for oxytocin (OT) mRNA expression (RT‐PCR) or for immunofluorescence (ir) staining with OT antibody (1:200.000). CBX abolished pressor and bradicardic responses to chemoreflex activation (KCN 40 μg, iv) in S and T rats. T induced increases in both OT mRNA (1.3±0.2 vs 2.6±0.4, ShamS vs ShamT) and OTir in PVN preautonomic subnuclei (dorsal cap, dc = 15±2 vs 23±1%; ventromedial, vm = 36±1 vs 44±1%; posterior, post = 9±1 vs 17±5 %, respectively). CBX did not change basal expression, but blocked T‐induced OT gene and protein expression (ShamT vs CBXT: PVN OT mRNA= 2.6±0.4 vs 1.4±0.6; OTir dc = 23±1 vs 15±2 %); OTir vm = 36±1 vs 22±3; OTir post = 17±2 vs 12±2%). Chemoreceptors are not tonic to drive preautonomic oxytocinergic neurons, but participate in the peripheral signaling to modulate T‐induced OTergic plasticity Financial Support: FAPESP, CNPq