Premium
The effects of Apelin‐13 on cardiovascular system can be mediated by modulating the excitability of SFO neurons
Author(s) -
Dai Li,
Smith Pauline M,
Ferguson Alastair V
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.889.3
Subject(s) - apelin , subfornical organ , microinjection , medicine , endocrinology , blood pressure , central nervous system , hypothalamus , homeostasis , circumventricular organs , osmoreceptor , chemistry , angiotensin ii , receptor
Apelin is an adipocyte derived hormone involved in the regulation of water balance, food intake, and the cardiovascular system via actions in the central nervous system. The subfornical organ (SFO) is a circumventricular organ with well document roles in body fluid homeostasis, cardiovascular control and more recently, energy balance. Owing to the lack of the normal blood‐brain barrier, SFO enables detecting circulating signal molecules from blood and cerebrospinal fluid. In this study, we have examined the effect of apelin on blood pressure (BP) and heart rate (HR) of anesthetized SD rats by microinjection of apelin into SFO in vivo and on the excitabilities of dissociated SFO neurons in vitro. We found that microinjection of apelin into SFO decreased BP (‐ 1492.3 ± 357.1 mmHg*sec, n=5) and HR (−32.4 ± 10.39 beats, n=5). Whole cell current clamp recording revealed that 100 nM aplein caused a hyperpolarization in 41 % of the tested SFO neurons (−9.9 ± 1.3 mV, n=21), while 33 % of neurons were depolarized by apelin administration (6.8 ± 0.9mV, n=17). Our research suggests that circulating apelin can directly affect BP and HR via modulating the excitability of SFO neurons.