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Ammonia and cortisol are transcriptional regulators of the ammonia transporter Rhcg2 in trout gill pavement cells
Author(s) -
Nawata Michele,
Wood Chris
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.886.14
Subject(s) - gill , trout , ammonia , transporter , biology , microbiology and biotechnology , protein kinase a , fish <actinopterygii> , gene , kinase , chemistry , biochemistry , fishery
Ammonia is the primary nitrogenous waste excreted across the gills of fish, but the mechanisms involved have never been clearly defined. Several very recent studies have now revealed that Rh glycoproteins have a role as ammonia transporters in fish gills. Based on gene expression studies, Rhcg2 appears to have a main role in this process in the pavement cells of the freshwater trout gill. Little is known however, about how these Rh genes are regulated. Using cultured trout pavement cells we have examined some of the possible transcriptional regulatory mechanisms of Rhcg2. We show that ammonia alone increases the level of Rhcg2 transcripts in the pavement cells while cortisol and 11‐deoxycorticosterone have a potentiating effect on this transcriptional response. We focus on cAMP and mitogen‐activated protein kinase pathways as potential signaling pathways involved in the regulation of Rhcg2 (NSERC Discovery).