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T lymphocytes infiltrating the kidney of Dahl SS rats are activated and differentiated
Author(s) -
Rudemiller Nathan,
Lund Hayley,
Guo Chuanling,
Flister Michael,
De Miguel Camen,
Mattson David L.
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.879.1
Subject(s) - kidney , spleen , medicine , endocrinology , cytokine , biology , immunology
Previous studies demonstrated that immunosuppression attenuates Dahl SS hypertension and renal disease (Am J Physiol 298:R1136). Experiments in this study examined the infiltration of T lymphocytes in the kidney and other organs and characterized the cytokine profile of the infiltrating cells in the kidney. No significant changes in T cell number were observed in the spleen, brain, blood, or aorta of Dahl SS rats fed the low (0.4% NaCl) or the high salt (4.0% NaCl) diet (n=4–7). A significant increase in T cells was observed in the liver (from 0.8±0.1 to 2.0±0.3 × 10 5 cells/gram) and in the kidney (from 3.1±0.6 to 6.2±0.9 × 10 5 cells/gram) of Dahl SS rats fed high salt. A gene expression analysis with real‐time PCR was performed on RNA isolated from circulating T cells and T cells isolated from the kidneys of 12 Dahl SS rats (4 pooled samples of 3 individual rats each) fed high salt for three weeks. The infiltrating T cells in the kidney expressed significantly greater (by 5–54 fold) mRNA for proliferation factors (Il‐2, Il‐4, and IFN‐γ) and for cytokines and genes related to Th1 cells (TNF, Il‐10, CCr5, Csf2), Th2 cells (Il4, Il6, Il10), and Th17 cells (Il‐6 and TGFβ) compared to circulating T cells. This study shows that the inflammatory state of T cells infiltrating the kidney, which could have implications in renal disease and hypertension, is starkly different from that of the peripheral T cells.