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Hyper‐caloric diet induces a hydrogen sulfide‐dependent mechanism in aortic perivascular adipose tissue (PVAT) function in Dahl S rats
Author(s) -
Spradley Frank T,
Ho Dao H,
Pollock Jennifer S
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.878.3
Subject(s) - medicine , endocrinology , aorta , chemistry , adipose tissue , cystathionine beta synthase , phenylephrine , blood pressure , enzyme , biochemistry , cysteine
PVAT‐mediated blunting of vasoconstriction (PVAT function) is reduced in some obese rodent models. Dahl salt sensitive rats (Dahl S) are predisposed to cardiovascular disease. We tested the hypothesis that hypercaloric diet (HCD; 59% fat/0.4% NaCl) promotes PVAT dysfunction in Dahl S. At 16 weeks old, PVAT mass was greater following a 4 week HCD compared to normal diet (ND; 14% fat/0.4% NaCl) (6.1±0.3 vs 1.9±0.3 PVAT weight:aorta weight; P<0.05; N=6). Using wire myography, phenylephrine (PE) mediated constriction was less in PVAT‐intact vs PVAT‐removed aortae from HCD Dahl S (62±11% vs 145±10% increase in force; P<0.05; N=5). Endothelium (endo) denudation did not alter PE response in PVAT‐intact aortae from HCD Dahl S. Inhibition of the hydrogen sulfide (H2S) generating enzyme cystathionine γ lyase (PAG; 10mM) increased PE response in PVAT‐intact, endo‐denuded aortae from HCD Dahl S (106±8% vs 74±12% increase in force; P<0.05; N=4). In ND Dahl S, PE response was less in PVAT‐intact compared to PVAT‐removed aortae (90±7% vs 157±9% increase in force; P<0.05; N=6), however endo denudation restored PE response in PVAT‐intact aortae (139±2% increase in force). In conclusion, PVAT from ND Dahl S does not directly modulate vascular smooth muscle tone in endo‐denuded aortae whereas a short‐term HCD elicits a functional response in PVAT from Dahl S via an H2S‐dependent mechanism. Funding: PO1 HL69999 and AHA 10PRE4010001.