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Combined effect of advanced glycation end products and physiologically relevant dynamic shear stress on endothelial cell functions
Author(s) -
Maria Zahra,
Yin Wei,
Rubenstein David Alan
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.876.9
Subject(s) - glycation , shear stress , thrombomodulin , albumin , chemistry , endothelial dysfunction , endothelial stem cell , endothelium , medicine , oxidative stress , stenosis , endocrinology , biophysics , cardiology , microbiology and biotechnology , diabetes mellitus , platelet , biochemistry , thrombin , biology , materials science , in vitro , composite material
Advanced glycation end products (AGEs) and disturbed shear stress are hallmarks of cardiovascular diseases. Our goal was to evaluate the combined effect of AGEs and physiologically relevant dynamic shear stress on endothelial cell (EC) functions. ECs were exposed to glycated or non‐glycated albumin for up to 5 days. ECs were then subjected to shear stress (SS) waveforms mimicking cardiac flow, stenosis flow and recirculation flow for 1hr. EC morphology, metabolic activity and shear induced actin alignment was observed. Also, the expression of ICAM, tissue factor and thrombomodulin was measured to quantify inflammatory and thrombogenic potential. We hypothesized that the combination of disturbed SS and AGEs would enhance EC responses to mimic endothelial dysfunction. Results indicate that ECs incubated with AGEs and exposed to stenosis SS had an altered actin alignment and structure. EC metabolic activity in the presence of glycated albumin, decreased when exposed to disturbed flow. Inflammatory and thrombogenic potential was also enhanced. Thus, the results indicate that the presence of AGEs severely alters EC function under various pathological SS conditions. Thanks to OCAST for supporting this work.