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Effect of Omega‐3 PUFA on Endothelin‐1 Receptors in Normal and Type 1 Diabetic Rat Hearts
Author(s) -
Bikhazi Anwar B,
El Chami Mohamad Imad,
Zwainy Nadine S,
Der-Boghossian Asdghig H
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.876.8
Subject(s) - medicine , endocrinology , endothelin receptor , receptor , endothelin 1 , diabetes mellitus , western blot , streptozotocin , statin , nitric oxide , chemistry , biochemistry , gene
Cardiovascular disease is a major complication in diabetes. Its management includes renin‐angiotensin‐system components, endothelial factors (Nitric Oxide), or Endothelin‐1 (ET‐1) regulation. This study focuses on long‐term ET‐1 receptor regulation by ω‐3 and Statin at coronary endothelium (CE) and cardiomyocytes (CM) levels in diabetic rats. Sprague Dawley male rats (n=24) were assigned to 7 groups: normal (N); streptozotocin (STZ)‐induced diabetic (D); normal rats treated with ω‐3 (0.085g/kg) (NO) or Statin (0.89 mg/kg) (NS); diabetic rats treated with ω‐3(DO), Statin (DS) or both (DOS). After a month of treatment, their hearts were perfused with [ 125 I]‐ET‐1 (CHAPS‐untreated for CE and CHAPS‐treated for CM analyses) to estimate ET‐1 receptor‐binding affinity (τ = 1/K−n) on CE and CM. Tissue samples were analyzed by western blot for ET‐1 receptor subtypes A (ET A R) and B (ET B R). Residency time constant, τ, of ET B R increased in all groups as compared to N (1.05 ± 0.06), except for DS (1.18 ± 0.07) which was normalized. As for ET A R, τ increased in all groups but normalized in DO (1.18 ± 0.07). In western blot, ET A R increased (1.31 ± 0.09 vs 0.96 ± 0.05) and ET B R decreased (1.00 ± 0.06 vs 1.30 ± 0.07) in D, but both values were corrected in all other groups. Thus, ω‐3 and Statin therapy improved ET A R and ET B R functions respectively in diabetics; however, ω‐3 dosage regimens needs further analysis in healthy subjects.