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Orthopedic trauma reduces renal hemodynamics in the STZ‐induced diabetic rat
Author(s) -
Manigrasso Michaele Beth,
Davis Deborah D,
Lu Silu,
Xiang Lusha,
Hester Robert L
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.876.5
Subject(s) - medicine , diabetes mellitus , renal function , hemodynamics , type 2 diabetes , endocrinology , urine , urology
Diabetes is an independent risk factor for the development of acute renal dysfunction following orthopedic trauma (OT). Whether OT affects renal hemodynamics has yet to be investigated. The hypothesis tested in this study was that OT reduces renal hemodynamics in type 1 diabetes. Male lean Zucker rats (12 wks) were divided into 4 treatment groups (n=4/gp): non‐diabetic without trauma (ND‐NT), non‐diabetic with trauma (ND‐T), STZ‐induced diabetic without trauma (D‐NT) and STZ‐induced diabetic with trauma (D‐T). Four wks after induction of diabetes, animals were implanted with catheters and OT was induced by soft tissue injury and local injection of bone components in both hind limbs. Animals were placed in metabolic cages for 12hrs to collect urine and urine albumin excretion (UAE) was measured by ELISA. 24 hrs post‐trauma, glomerular filtration rate (GFR) was measured in conscious rats. Our results show that diabetes after 4 wks causes a reduction in GFR compared to controls. However, OT caused a greater reduction in GFR in diabetics than controls. Our data suggest that OT compromises renal function in the setting of type 1 diabetes.Group Body Weight (g) Glucose (mg/dL) UAE (mg/day) GFR/KW (ml/min/g)ND‐NT 396±25 81±6 0.5±0.1 1.37±0.09 ND‐T 415±20 85±4 1.2±0.7 1.52±0.08 D‐NT 298±12 * # 358±27 * # 3.3±0.6 * # 1.17±0.08 # D‐T 330±14 * # 366±15 * # 4.6±0.3 * # 1.06±0.07 * #Avg±SEM;* P<0.05 vs. ND‐NT;# P<0.05 vs. ND‐T