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The genetic association of polymorphisms rs599839, rs646776 and rs4970834, in chromosomal locus 1p13.3 with coronary artery disease
Author(s) -
Rizk Nasser,
El-Menyar Ayman,
Wais Idil Souleman,
Egue Huda,
Baluli Hissa Mohamed,
Al Suwaidi Jassim
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.874.19
Subject(s) - coronary artery disease , medicine , genotyping , diabetes mellitus , gastroenterology , genotype , cardiology , endocrinology , genetics , biology , gene
Background Abnormalities in lipids with increased serum concentration of LDL‐C are risk factors for coronary artery disease (CAD), and atherosclerosis. The aim of this study was to investigate the association between serum LDL‐cholesterol, and significant coronary artery stenosis with well‐known genetic variants in chromosomal locus 1p13.3 among CAD patients with and without significant coronary artery stenosis [case and control groups] respectively. Methods Blood was drawn from a total of 178 patients. Genotyping for the variants rs599839 , rs646776 and rs4970834, were performed by the 5’ nuclease test using TaqMan assay. Results The HDL‐C level of rs599839 was [12.01± 3.9 mM vs. 3.46± 1.45 mM] for GG vs. AA+AG using the recessive genetic model with p value=0.006. Using the additive model, rs64776 variant had a significantly higher triglycerides levels [7.76±2.25 vs. 1.74±0.15 mM] for AA vs. GG respectively with p value=0.031. No significant difference was observed for LDL‐C and cholesterol among genotypes of all variants using different genetic models. The rs599839 variant had a significant association with the severity of coronary stenosis using additive model. The OR was 2.7 times among patients with significant stenosis (p =0.04). Stepwise regression analysis revealed that the following independent variables have a statistically significant effect on LDL‐C level; BMI, diabetes, TC, TG, HDL‐C, and anti‐lipidemic treatment in presence of rs59839 and rs646776 and rs4970834. Conclusion In summary, polymorphism rs646776 and of rs599839 were significantly associated with HLD‐C and triglycerides levels, but not with LDL‐C. In addition the variant rs599839 had a significant association with atherosclerotic lesion in coronary vessels.