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Potential Proteomic Biomarkers of Secondhand Smoking‐ Induced Cardiovascular Disease
Author(s) -
Zweier Jay L.,
Tewari Arun K,
El-Mahdy Mohamed A,
Abdelghany Tamer M,
Hemann Craig,
El-Sherbiny Gamal A.
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.874.13
Subject(s) - haptoglobin , fibrinogen , ceruloplasmin , medicine , immunology , disease , oxidative stress , apolipoprotein b , blood proteins , secondhand smoke , coagulation , pathology , endocrinology , cholesterol , environmental health
Identification and characterization of proteomic changes that can serve as predictive markers of the onset and progression of secondhand smoke (SHS)‐induced disease are now in the forefront of cardiovascular (CV) research. Our objective was to identify a differential pattern of protein expression in the plasma of SHS‐exposed mice through a time course study. C57BL/6 mice were exposed for up to 48 weeks to SHS generated from 3R4F reference research cigarettes using the Teague smoking machine. Utilizing 2D‐DIGE and MS/MS, we have found a differential expression of 35 proteins in SHS‐exposed mice as compared to controls. Some of those are involved in vascular function, coagulation, or metabolism. Expression of ceruloplasmin, haptoglobin, fibrinogen, apolipoprotein E and murinoglobulin was ~2.5, 7, 3, 2 and 3.5‐fold lower than controls, respectively. The expression of alpha‐1 antitrypsin and complement C3 was ~6 and 13‐fold higher than controls, respectively. The alterations in fibrinogen, alpha‐1 antitrypsin, haptoglobin, and complement C3 are of particular interest since these have been linked to CV and lung pathology mediated by oxidative stress. Our data suggest that SHS exposure leads to changes in plasma protein expression. These changes can potentially serve as predictive markers of the onset and/or temporal progression of CV and pulmonary disease in secondhand smokers. (NIH # HL63744 to JLZ)