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Model of pulmonary hypertension induced by reversible pressure‐overloaded left heart failure in rats
Author(s) -
Chovanec Milan,
Herget Jan
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.873.23
Subject(s) - medicine , cannula , pulmonary artery , pulmonary hypertension , cardiology , heart failure , ascending aorta , aorta , surgery
We focused on pulmonary hypertension induced by left heart failure in rats caused by inserting a polyethylene cannula into the ascending aorta via the left carotid artery. In experiment A, an obturating cannula was implanted for 1 week (group 1wk, n=7), for 3 weeks (group 3wks, n=6) and for 3 weeks with a 3 weeks recovery period after removal of the cannula (group 3+3wks, n=8). We measured RV/LV+S ratio. We found no difference in heart weight vs. controls in the 1wk and 3+3wks groups. The RV/LV+S ratio (0.39±0.024 vs. 0.27±0.008; p<0.01) was significantly increased in the group 3+3wks compared to controls. In experiment B, we measured pulmonary artery pressure (PAP) in closed‐chest animals. Group N (n=8) were normoxic controls. Group H (n=8) were hypoxic controls. Rats were exposed to normobaric hypoxia (10% O 2 ) for 3 weeks. Mean PAP was significantly elevated in groups 1wk and 3wks compared to normoxic controls (25.0±1.18mmHg and 22.9±0.66mmHg vs. 16.9±0.97mmHg; p<0.01 and p<0.05 respectively) but significantly lower compared to hypoxic controls (25.0±1.18mmHg and 22.9±0.66mmHg vs. 33.4±2.0mmHg; p<0.001 and p<0.0001 respectively). This is a simple, well tolerated and fully reversible method of inducing pressure‐overloaded left heart failure followed by the significant pulmonary hypertension. Supported by: GACR 305/05/08/108, GAUK 78007 and Cardiovascular Research Centre 1M510.