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Morphological characterization of intrinsic cardiac nervous system in left ventricular hypertrophy
Author(s) -
Gomez Maria,
Reynoso Alejandro,
Giles Ruth,
Flores Gonzalo
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.872.7
Subject(s) - endocardium , ventricle , ganglion , muscle hypertrophy , medicine , left ventricular hypertrophy , anatomy , cardiac ventricle , chemistry , blood pressure
The intrinsic cardiac nervous system (ICNS) is organized in ganglion connected by nerve fibers. Little information about their changes in heart hypertrophy is available. In this study we characterized the morphological changes of the ICNS in hearts with experimental hypertrophy with the Golgi‐Cox and Glyoxylic acid methods. Hearts from Spontaneous Hypertension (SH) and Wystar Kyoto (WK) rats at 4 and 8 months age were processed. Hearts were extracted, washed, and incubated for 30 days in Golgi Cox solution, sectioned by vibrotome (200 microns), processed and analyzed by the Sholl method under light microscopy adapted to a camera lucida. Diameters of ganglion and nerve fiber from endocardium, myocardium and epicardium from left ventricles were recorded in microns. Histofluorescence for catecholamine at 2 % was made and analyzed qualitatively. Data are expressed as mean ± SEM and analyzed with t‐test , P < 0.05 was considered significant. Clearly increase in number and size of ganglion and nerve fibers was observed in the left ventricle from SH rats. Ganglion are increasing in size from epicardium to endocardium (51.8±5.4, to 114.1± 8.8 micron) in WK and in SH rats, the ganglion size were higher, particularly in endocardium (66.30±7.79, and 138± 14, respectively). Histofluorescence for catecholamine in left ventricle hypertrophy was increased. Ours results demonstrate increases in size and number of nerve structures in left ventricle hypertrophy, and suggest that the adaptative tissular changes generated in hypertrophy also involve the intrinsic cardiac nerve system.