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The Effects of Captopril Treatment and Exercise Training on Blood Pressure and Endothelium Function in the Spontaneously Hypertensive Rat
Author(s) -
Woods Vernikka,
Coleman-Shepherd Gwendolynn,
Runnels James,
Keaton Alphonso
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.872.37
Subject(s) - captopril , isometric exercise , medicine , blood pressure , angiotensin converting enzyme , endothelium , endocrinology , serotonin , contraction (grammar) , spontaneously hypertensive rat , receptor
Angiotensin converting enzyme inhibitors (ACE‐I) have been show to prevent impairment of endothelial cell function in the Spontaneously Hypertensive Rat (SHR). The purpose of this study was to examine the combined effects of long‐term exercise training and ACE‐I treatment on blood pressure and vascular reactivity in the SHR. Four groups of male SHR were studied: 1) untreated SHR; 2) exercise trained (35 cm/s) for 60 minutes/day, for 5 days/week (SHREX); 3) captopril treated (50 mg/kg/day), treatment was initiated at four weeks of age and continued for 12 weeks (CAP); and 4) captopril treated + exercise (CAPEX). N = 8, for all four groups. All rats were studied at 16–18 weeks of age. Mean arterial pressure (MAP) in the untreated SHR was 130±5 mmHg. Exercise treatment in the SHR significantly decrease MAP in the (SHREX – 114±7 mmHg). Oral administration of captopril was associated with a significant decrease in MAP (97±3 mmHg) compared to untreated SHR and SHREX. Further, captopril treatment + exercise significantly lowered MAP in the CAPEX group (107±6mmHg, vs. SHR). Isolated aortic ring segments were suspended in tissue chambers for measurement of isometric force. Ring segments were exposed to cumulative concentrations of serotonin (3× 10 −9 − 3 × 10 −5 M). Aortic rings from SHR demonstrated increased responsiveness to serotonin induced contraction in comparison to aortic rings from the CAP and CAPEX group. Endothelium‐dependent relaxation to acetylcholine was dramatically impaired in the SHR. Endothelium‐dependent relaxation was markedly greater in both the CAP and CAPEX group. These findings suggest that early long‐term treatment of captopril coupled to exercise training can prevent alterations in endothelial function observed in the SHR….