Premium
Losartan abolished hyperglycemic effect of chronic mild and unpredictable stress in rats
Author(s) -
Marcondes Fernanda Klein,
Sanches Andrea,
Costa Rafaela,
Cunha Tatiana Sousa,
Casarini Dulce Elena
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.869.20
Subject(s) - losartan , endocrinology , medicine , insulin , angiotensin ii , receptor
We've shown previously that chronic mild and unpredictable stress (CMUS) induced endothelium dysfunction and increased renin‐angiotensin system (RAS) activity in rats. Since endothelial dysfunction and high RAS activity has been associated with insulin resistance the aim of this study was to evaluate the effect of losartan on the response to oral glucose tolerance test (OGTT) of rats submitted to CMUS. Male Sprague‐Dawley rats were divided into four groups: control, CMUS, losartan (50mg/Kg) and CMUS + losartan (n=12/group). CMUS protocol consisted in applying different stressor stimuli (immobilization, overnight illumination, water and food deprivation, wed bedding and reversed light/dark cycle) 7 days/wk during three consecutive weeks (from the 3rd to 5th wk) of the experimental protocol, 7 weeks long. OGTT has been performed one week after CMUS. Data were analysed by ANOVA bifatorial (p<0.05). All procedures were approved by Institutional Ethics Committee. CMUS and CMUS+losartan groups presented higher corticosterone plasma level in comparison with non stressed groups. CMUS group presented higher area under the curve of glucose during OGTT in comparison with control group (885,2 ± 10,07 vs. 754,4 ± 16,08 mmol x min/L), without difference between losartan (745,9 ± 15,40) and CMUS+losartan group (816,4 ± 17,01 mmol x min/L). These data show that increased RAS activity is involved in the CMUS hyperglycemia.