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Augmented coronary vasoconstriction to epicardial perivascular adipose tissue in metabolic syndrome
Author(s) -
Kohr Meredith,
Lai Xianyin,
Moberly Steven P,
Berwick Zachary C,
Witzmann Frank A,
Tune Johnathan D
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.866.11
Subject(s) - adipose tissue , medicine , endocrinology , vascular smooth muscle , vasoconstriction , coronary arteries , metabolic syndrome , downregulation and upregulation , cardiology , epicardial adipose tissue , artery , chemistry , smooth muscle , obesity , biochemistry , gene
This investigation was designed to delineate the mechanisms and potential adipocytokine factors by which epicardial perivascular adipose tissue (PVAT) influences coronary smooth muscle function in the setting of the Metabolic Syndrome (MetS). We performed isometric tension studies on isolated coronary arteries from lean and MetS Ossabaw swine before and after the addition of PVAT to the tissue baths. The addition of PVAT (0.1 to 1.0g) increased active tension development in both lean and MetS swine. This effect was augmented with MetS PVAT (~2‐fold at 0.3g) and dependent on the quantity of PVAT added to the bath. Interestingly, active tension development of arteries pre‐constricted with KCl (20 mM) or PGF2α (10μM) was further amplified by the addition of PVAT from both lean and MetS swine. PVAT‐mediated increases in active tension development were similar in endothelium intact vs. denuded arteries and were reversed by diltiazem (10μM). LC‐MS/MS of lean and MetS coronary PVAT supernatants revealed 41 proteins significantly upregulated and 51 downregulated by the MetS. These data indicate that epicardial PVAT predominantly produces vasoconstrictor factors that act via Cav1.2 channels in vascular smooth muscle. Differential protein expression in the PVAT may be responsible for augmented vascular responses in the MetS.