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Viability of two sub‐strains of C57BL/6 mice in response to cardiac pressure‐overload
Author(s) -
Parikh Jay,
Wang Sophia,
Azizi Khalid,
Bird Elizabeth,
Spellman Matthew,
Lai N. Chin
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.864.6
Subject(s) - contractility , pressure overload , medicine , cardiology , cardiac function curve , blood pressure , heart rate , strain (injury) , constriction , heart failure , cardiac hypertrophy
The inbred mouse strain C57BL/6 is highly suitable and commonly used for the generation of transgenic mice as tools in dissecting biological processes and diseases. However, this parent line is available from a variety of sources with different sub‐strains. We examined male C57BL/6J mice from the Jackson Laboratory (n=29) and male C57BL/6NHsd from the Harlan Laboratories (n=36) in the setting of transverse aortic constriction (TAC), which is a common interventional procedure for studying pressure‐overload in hearts. Under anesthesia, the aortic arch between the innominate and the left carotid artery was constricted to 0.413 mm (27 ga needle) with a 7‐0 silk suture. Survival was monitored for 3 wks, and subsequently left ventricular function was assessed with a 1.4F Millar pressure transducer. Three wks after TAC, the survival rate was 50% for the C57BL/6NHsd and 79% survived for the C57BL/6J (Top Figure). A significant decline of 1058 mmHg/s (21%) in cardiac contractility (Bottom Figure) and 1228 mmHg/s (22%) in the rate of decay was found in C57BL/NHsd mice. Conclusion This study demonstrates phenotypic differences in viability and cardiac performance in response to TAC for these two sub‐strains. Therefore, it is imperative to carefully consider the appropriate line for the design of transgenic mice.