Premium
Endothelial bioeffects from acoustic droplet vaporization for gas embolotherapy
Author(s) -
Bull Joseph L,
Seda Robinson,
Samuel Stan,
Fowlkes J. Brian
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.859.14
Subject(s) - microbubbles , cavitation , cremaster muscle , vaporization , jet (fluid) , in vivo , materials science , microcirculation , mechanics , chemistry , biomedical engineering , biophysics , ultrasound , acoustics , physics , biology , medicine , organic chemistry , microbiology and biotechnology , radiology
The dynamics of vascular microbubbles within the microcirculation is examined using a combination of experiments and theoretical analyses. This work is motivated by a developmental gas embolotherapy technique for cancer treatment by infarction of tumors with selectively formed gas bubbles. Additionally, the transport component of this work is relevant to air embolism. The bioeffects resulting from acoustic droplet vaporization (ADV) of a superheated dodecafluoropentane microdroplet is investigated using endothelial cells cultured in Opticells and in vivo in the rat cremaster muscle. The subsequent transport and lodging of microbubbles are examined in the cremaster. Acoustic pressures sufficient for ADV, but below the threshold for inertial cavitation of the resulting bubbles results in smaller damage regions in the Opticell experiments. The onset of inertial cavitation results in a larger region of endothelial bioeffects. The occurrence of bioeffects in the in vivo studies is found to depend on the size vessel in which the droplets are vaporized, with smaller vessels (relative to the initial droplet radius) leading to larger bioeffects. The subsequent lodging of the bubbles agrees with predictions from our mathematical models. This work is supported by NIH grant R01EB006476.