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Computational Analysis of a Microfluidic Device for Measuring Oxygen‐Dependent ATP Release from Erythrocytes
Author(s) -
Sove Richard,
Ghonaim Nour W.,
Ellis Christopher G.,
Goldman Daniel
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.859.13
Subject(s) - adenosine triphosphate , luciferin , biophysics , atp synthase , chemistry , microfluidics , oxygen , kinetics , red blood cell , oxygen transport , biochemistry , nanotechnology , materials science , biology , enzyme , luciferase , physics , transfection , organic chemistry , quantum mechanics , gene
Release of adenosine triphosphate (ATP) from erythrocytes is believed to be a key component of the system for regulating microvascular blood flow. Recently, shear rate‐dependant ATP release from erythrocytes was measured using a microfluidic device (Wan et al, PNAS, 2008). These authors found ATP release occurred within 25–75ms following increased shear. The present work seeks to apply a similar approach to oxygen‐dependant ATP release, the dynamics of which are thought to be important in regulation of microvascular O2 delivery. Our computational model allows for the analysis of such a system to: i. design the optimal device to measure ATP release and ii. quantitatively interpret the resulting output signal. A computational model was constructed based on hemodynamics, convective‐diffusive transport of O2 and ATP, ATP/luciferin‐luciferase reaction kinetics, and optics. The computational model shows erythrocyte O2 levels can be altered rapidly enough to measure a delay in ATP release of 25ms. We show that this computational model is appropriate for analyzing properties associated with erythrocyte ATP release including the relationship between hemoglobin O2 saturation and magnitude of ATP release.