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Perivascular resident macrophages in the inner ear are essential for blood‐labyrinth‐barrier integrity
Author(s) -
Shi Xiaorui
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.855.10
Subject(s) - tight junction , vascular permeability , blood–brain barrier , basal lamina , inner ear , in vivo , microbiology and biotechnology , chemistry , biology , ultrastructure , pathology , anatomy , medicine , neuroscience , central nervous system
The inner ear has an endothelial blood‐tissue barrier in the stria vascularis that is as tight as the blood brain barrier. However, the mechanisms that control stria vascularis endothelial blood‐barrier permeability remain largely unknown. In this study, we provide the first evidence that signaling between perivascular resident macrophages (PVMs) and endothelial cells modulates blood‐labyrinth barrier (BLB) permeability. Morphological study of these cells with transmission electron microscopy showed a rich network of PVMs’ processes contacted capillary by an electron dense basal lamina. Their end‐feet of the processes were strikingly rich in mitochondria and were heterogenous in size and shape. The ending of processes of PVMs also surprisingly contained numerous vesicles. The functional study for PVMs in vitro and in vivo showed that PVMs are critical cellular components for blood‐labyrinth integrity as ablation of PVMs in a transgenic mouse model, utilizing targeted dyptheria receptor method, caused vascular leakage by affecting the formation of tight junctions mediated by pigment epithelial‐derived factor. This work was supported by National Institutes of Health grants NIDCD DC008888, NIDCD DC008888S1, NIDCD DC010844, NIDCD DC000105, P30 DC005983.