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Hemodynamic parameters during acute and chronic metabolic acidosis in rabbits
Author(s) -
Celotto Andrea Carla,
Ferreira Luciana Garros,
Capellini Verena Kise,
Albuquerque Agnes Afrodite Sumarelli,
Evora Paulo Roberto Barbosa
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.853.29
Subject(s) - metabolic acidosis , acidosis , hyperventilation , medicine , hemodynamics , endocrinology , cardiac output , bicarbonate , pco2 , blood pressure , anesthesia
Metabolic acidosis has profound effects on vascular tone, with impact on systemic and local flow and blood pressure (BP) control. This study investigated the effect of in vivo acute and chronic metabolic acidosis on hemodynamic parameters. Chronic metabolic acidosis (CMA) was induced by intake of NH4Cl 1% during 7 days ( ad libidum ), while acute metabolic acidosis (AMA) was induced by infusion of NH4Cl 6M (1ml/kg, diluted 1:10, during 3h). Dose‐response curves to Phe and ACh were performed in acidotic animals during venous and arterial BP monitoring to an indirect evaluation of vascular response. In the CMA (pH 7.15 ± 0.03) the bicarbonate reduced (13.8 ± 0.98 mmol/L) without changes on pCO2. In the AMA (pH 7.20 ± 0.01) bicarbonate decreased (10.8 ± 0.54 mmol/L) and pCO2 decreased (47.8 ± 2.54 to 23.2 ± 0.74mmHg) with simultaneous hyperventilation. No difference on arterial or venous BP was observed during Phe or ACh infusion for CMA, while for AMA the ACh infusion promoted greater arterial BP decreased (ΔBP: −28.0 ± 2.35 AMA to −14.5 ± 2.89 control mmHg). No changes on cardiac frequency or cardiac output were observed. The NOx plasma levels were normal in CMA, but were increased after AMA (25.3 ± 0.88 to 31.3 ± 0.54μM). These results point that AMA, but not CMA, was able to potentiate the ACh effect on BP accompanied by NOx increases, reinforcing the effect of pH imbalance on vascular tone and BP control. Support: FAPESP; FAEPA‐HC/FMRP.