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THE EFFECT OF NORMOBARIC OXYGEN ON THE INJURY INDUCED BY FOCAL CEREBRAL ISCHEMIA IN RATS VIA HIF‐1‐NOTCH SIGNAL PATHWAY
Author(s) -
chen chunhua,
Zhang Weiguang,
Yang Lei,
Wang Ke,
zhou Changman
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.852.5
Subject(s) - nissl body , western blot , medicine , ischemia , natural bond orbital , immunohistochemistry , anesthesia , staining , pathology , pharmacology , chemistry , biochemistry , density functional theory , computational chemistry , gene
Objective To investigate the effect of pretreatment of inhalation of normobaric oxygen(NBO) on the injury induced by focal cerebral ischemia via HIF‐notch pathway. Methods Male SD rats were randomly divided into four groups: Control (sham surgery), Middle Cerebral Artery Occlusion and Reperfusion(MCAO), MCAO pretreated with Normobaric Oxygen (NBO), MCAO with NBO injected with 2ME2. 2ME2 was injected intraperitoneally at 1h before the inhalation of NBO. NBO was inhaled for 4h a day and for 6d continuous. We observed the neurological deficits in different groups. Rats were sacrificed at 24h for TTC staining, western blot, nissl and immunohistochemical staining. Results NBO pretreatment improved neurological deficits, reduced the infarct volume (P<0.05), inhibited the neuronal deformation shown by nissl staining and increased the expression of HIF‐1¦Á and Notch1 detected by Western Blot and immunohistochemical staining. But all the improvement were lessened by the 2ME2 injection. Conclusion NBO pretreatment may protect brain from cerebral ischemic injury via HIF‐Notch1 signal pathway.