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The Potential of a Cellulose Nanocrystal and Water Soluble Chitosan Complex as an Oral Drug Delivery Carrier
Author(s) -
Colacino Katelyn Rose,
Wang Hezhong,
Hong Jung Ki,
Roman Maren,
Lee Yong Woo
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.851.13
Subject(s) - occludin , chitosan , chemistry , polysaccharide , drug delivery , permeability (electromagnetism) , polyelectrolyte , monolayer , caco 2 , cellulose , surface charge , drug carrier , tight junction , nanocrystal , biophysics , dissolution , chemical engineering , nuclear chemistry , nanotechnology , in vitro , materials science , biochemistry , membrane , organic chemistry , biology , polymer , engineering
The present study evaluates the potential use of polyelectrolyte‐macroion complexes (PMCs) between water soluble chitosan (WSC), a cationic polysaccharide, and cellulose nanocrystals (CNCs), anionic, rod‐like nanoparticles, as carriers in oral drug delivery applications. CNCs were prepared by sulfuric acid hydrolysis of dissolving pulp and carboxylated to increase surface charge. PMCs were prepared with WSC purchased from Qingdao Olympic Chemistry Company at a 1:1 charge ratio. Human epithelial colorectal adenocarcinoma (Caco‐2) cells were purchased from American Type Culture Collection and employed for measurements of transepithelial electrical resistance (TEER) and differences in tight junction protein expression. Field‐emission scanning electron microscopy images showed PMCs to be spherical structures with a diameter in the micron range. WSC and PMC treatment significantly decreased TEER across the Caco‐2 cell monolayer in a similar fashion. Additionally, immunofluorescent staining revealed the expression of tight junction proteins such as ZO‐1 and occludin significantly decreased after treatment with WSC and PMCs. These data provide evidence that WSC‐CNC PMCs alter TEER and permeability across Caco‐2 cell monolayers similarly to WSC. Our results suggest that WSC‐CNC PMCs are a viable option as carriers for oral drug delivery. (This work was supported by NSF # DMR0907567)