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The Effects of Site of Surgical Implantation on the Delivery Profiles of Anti‐Trypanosomal Agent (DFMO)
Author(s) -
Benghuzzi Hamed A,
Tucci Michelle,
Cameron Joseph A
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.835.14
Subject(s) - capsule , delivery system , medicine , surgery , chemistry , biomedical engineering , biology , botany
This investigation was designed to compare the interface zone of the tricalcium phosphate lysine delivery devices loaded with diflouromethyl ornithine (TCPL‐DFMO) that were implanted either I/P or S/C. A total of 120 adult male rats were randomly divided into four groups. Each rat in groups 1–2 were implanted with TCPL‐DFMO devices I/P and S/C respectively and group 3 animals were implanted with empty TCPL (sham). Group 4 animals served as intact controls. At the end of 1, 4 and 10 weeks postimplantation10 animals from each were sacrificed following standard lab protocols and capsules were retrieved and blood analysis were performed. The results indicated that regardless of the site of surgical implantation there was a fibrous capsule which encompasses the implanted delivery system, and the presence of inflammatory cells were evident after the first week of implantation. The capsules implanted I/P experienced a greater portion of tissue growth and a higher release profile of DFMO (5–8 ng/ml/day) than those implanted S/C (2.7–4.8 ng/ml/day). In addition, the weights of the capsule and the weight of the tissue retrieved from S/C implants were significantly less than those implanted I/P. This information is necessary for future designs of the delivery devices, so that the prolonged delivery of DFMO from the TCPL is maximized.