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L‐Glutamine Enhances Wound Healing in Human Colonic Epithelial Cells
Author(s) -
Benton Shana,
Hao Li,
Merlin Didier,
Laroui Hamed,
Ziegler Thomas R.
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.833.5
Subject(s) - wound healing , glutamine , caco 2 , cell , endogeny , chemistry , cell growth , pharmacology , medicine , biochemistry , immunology , amino acid
Glutamine (GLN) administration has a variety of cytoprotective effects in intestinal epithelia but little data on GLN in wound healing are available. We studied the effect of L‐GLN on wound healing in human colonic Caco‐2 epithelial cells. Methods Wound injury was induced in Caco‐2 cell monolayers using electric cell substrate impedance sensing (ECIS) technology. At injury, concentrations of L‐GLN ranging from zero to 0.5 mM were added to cell medium ± L‐methionine sulfoximine (MSO), an inhibitor of glutamine synthetase. Continuous resistance (the index of wound healing) and cell permeability measurements were obtained for 38 hrs after cell injury. Results In the absence of MSO, low concentrations of L‐GLN increased Caco‐2 cell resistance/wound healing (0.1mM: P<0.01; 0.3mM: P<0.001; 0.5mM: P<0.001) in a dose‐response manner and decreased cell permeability vs control non‐GLN‐treated cells (P<0.05). In the presence of MSO, only the higher 0.5 mM GLN concentration improved wound healing (P<0.05) and decreased cell permeability compared to control (P<0.05). Conclusions After a standardized wound , L‐GLN improves wound healing and decreases cell permeability in human colonic epithelial cells; exogenous L‐GLN rescues these cytoprotective effects in the absence of endogenous GLN synthesis. Supported by NIH grants K24 RR023356 and T32 DK007298.