Premium
Black Raspberry Powder Inhibits IL‐8‐Mediated Angiogenesis in Human Intestinal Microvascular Endothelial Cells Role of; MAPK, Bcl2, Akt and COX‐2
Author(s) -
Link Benjamin,
Medda Rituparna,
Nie Linghui,
Schmidt Jamie,
Jovanovic Nebosja,
Otterson Mary F,
Rafiee Parvaneh
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.832.10
Subject(s) - angiogenesis , protein kinase b , cancer research , apoptosis , mapk/erk pathway , inflammation , chemistry , immunology , signal transduction , microbiology and biotechnology , biology , biochemistry
BACKGROUND Angiogenesis, the growth of new blood vessels, plays an important role in gastrointestinal chronic inflammation and cancer. Previously we have shown that interleukin 8 (IL‐8) exerts a potent angiogenic effect on human intestinal microvascular endothelial cells (HIMEC). Black raspberry (BRP) is a dietary product and an effective chemopreventive agent in animal models of malignancy, undergoing evaluation for the treatment of inflammatory bowel disease (IBD). However, the effect of BRP on HIMEC is not well defined. We investigated the antiangiogenic effect of BRP on IL‐8 activated HIMEC. METHODS HIMECs were treated with IL‐8 and BRP (5–50 μg/ml). Effect of BRP on survival/apoptosis, proliferation, transmigration, stress fiber and tube formation in IL‐8 activated cells were examined. The intracellular signaling molecules; MAPKs, Akt, Bcl2, Bax, NFκB and COX‐2 were assessed with or without BRP by Real‐time, Western blot and Immunofluorescence microscopy. RESULTS BRP inhibited IL‐8‐induced in vitro tube formation, cell migration, proliferation and stress fiber formation. IL‐8 enhanced Bcl2, Akt, COX‐2 expression and ERK1/2 and JNK activation. BRP down regulates Bcl2, Akt, ERK1/2, JNK, and COX‐2 expression and enhanced Bax expression in HIMEC. CONCLUSIONS Taken together, BRP possesses antiangiogenic properties, which warrants further investigation as adjuvant treatment of IBD and cancer.