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Comparison of diets containing whole grain oats versus low bran oat flour on insulin sensitivity and fecal microbiota composition in C57BL/6J mice
Author(s) -
Lefevre Michael,
Hergert Nancie,
Rompato Giovanni
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.830.8
Subject(s) - food science , bran , population , biology , insulin , composition (language) , feces , unifrac , zoology , chemistry , endocrinology , medicine , biochemistry , microbiology and biotechnology , raw material , 16s ribosomal rna , ecology , linguistics , philosophy , environmental health , gene
Our objective was to identify novel physiological effects of whole grain oat flour. Two AIN93G‐based diets were prepared containing 32% of energy from carbohydrate derived from either whole grain (WGO) or low bran (LBO) oat flour. Diets were fed to C57Bl/6J mice (13 mice/diet) for eight weeks. Weight gain, fat mass gain and food intake did not differ between the groups. Insulin sensitivity was greater in the WGO group as reflected by significantly (P<0.05) lower plasma levels of insulin, C‐peptide and resistin along with a trend for lower glucose excursions following intraperitoneal glucose injection. Cecal microbiota population structure was determined by pyrosequencing of the 16S rRNA gene. Similarity of microbiota populations was assessed by unweighted UniFrac analysis (which is sensitive to microbiota community membership) followed by principal coordinate analysis (PCoA). Plots of PCoA vectors 1 and 3 revealed near complete segregation of the populations by diet. PCoA vectors 1 and 3 were significantly correlated with levels of insulin, C‐peptide and resistin, and combined accounted for 47%, 39% and 48% of their plasma variation, respectively. These data suggest that improvements in insulin sensitivity with WGO are independent of changes in body composition and may be mediated through changes in cecal microbiota population composition. Funded by the General Mills Bell Institute of Health and Nutrition.