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Whey based formula reduces intestinal atrophy and enhances antioxidant defenses against sepsis
Author(s) -
Tsutsumi Rie,
Sasaga Sayaka,
Takegawa Mariko,
Sakai Tohru,
Nakaya Yutaka,
Tsutsumi Yasuo M.
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.825.5
Subject(s) - whey protein , antioxidant , sepsis , glutathione , atrophy , medicine , oxidative stress , chemistry , endocrinology , pharmacology , biochemistry , enzyme
High quality whey protein has been shown that improve anabolism, GI tolerance, and antioxidant defenses. In this study, we assess the effect of whey based formula during the acute phase in the cecal ligation and puncture (CLP) mousemodel of sepsis. Male C57Bl/6 mice began whey based or casein based formula following induction of sepsis by CLP. Between 48 h to 7 days of sepsis, survival rate, intestinal histopathology, intestinal atrophy, antioxidant defense and serum levels of cytokines were assessed. Analyses were performed with StatView 5.0 (SAS Institute Inc., Cary, NC). Data are presented as mean±standard deviation (SD). P<0.05 was considered statistically significant. Serum cytokines were higher in mice that underwent whey based formula vs control (TNFα;34.3±6.54 vs.67.6±8.93pg/ml, IL6;230+37 vs.443+49 pg/ml after 48 h induction, respectively). Whey‐based group showed re‐established total hepatic glutathione and glutathione disulfide (GSH/GSSG)levels. Whey‐based formula significantly improved intestinal atrophy which was measured by diamine oxidase (DAO) activity. Our data indicate that administering whey‐based formula after sepsis has several benefits by decreasing cytokines, increasing antioxidants and reducing intestinal atrophy.