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Association Between Single Nucleotide Polymorphism Ala‐9‐Val of Manganese‐Superoxide Dismutase and Normal Weight, Overweight and Obesity in Mexican Population
Author(s) -
Hernandez-Guerrero Cesar,
Parra-Carriedo Alicia,
Isoard-Acosta Fernando,
Perez-Lizaur Ana Bertha,
De Noriega Daniela Galindo,
Cherem-Cherem Loren
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.824.7
Subject(s) - overweight , obesity , single nucleotide polymorphism , body mass index , population , medicine , snp , genetics , biology , genotype , gene , environmental health
The study included Mexican participants with at least two generations of relatives born in Mexico. The control and study groups were selected from the Clinic of Nutrition of Health Department of the Universidad Iberoamericana of Mexico City. The control group included 36 participants with a body mass index (IMC) of: 18.5–24.9. The overweight group included 18 participants with IMC of: 25.0–29.9, and the obesity group included 29 participants with IMC >30. DNA was isolated by DNAzol reagent. The identification of SNP Ala‐9‐Val was done by Polymerase Chain Reaction technique using specific primers, follows of Restriction Fragment Length Polymorphism technique using the Bsa WI restriction enzyme. The frequency of polymorphism was analyzed by Xi squared and a p<0.05 was accepted as a statistical difference. The frequencies of SNP Ala‐9‐Val (homozygote plus heterozygote) were 0.34, 0.50 and 0.66 in control, overweight and obesity groups, respectively. There was a statistical difference between obesity and control group (Xi 2 = 5.44; p=0.019; O/R= 3.8; I.C. 95% 1.35 − 10.6). When the obesity group was stratified by obesity degree a statistical difference was observed between obesity‐III (IMC> 40) and control group (Xi 2 = 4.49; p=0.034; O/R= 5.0; I.C. 95% 1.29–19.3). The polymorphisms Ala‐9‐Val could be one of several genetic factors involved in the susceptibility to development obesity in the Mexican population.