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Gene expression profiles of the colonic mucosa associated with phenotypic changes in mice fed high‐fat diet
Author(s) -
Bae Yun Jung,
Bak Youn-Kyung,
Park Taesun,
Choi Myung-Sook,
Kim Jeongseon,
Sung Mi-Kyung
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.824.4
Subject(s) - biology , endocrinology , medicine , leptin , phenotype , adipose tissue , gene expression , adipokine , gene , obesity , genetics
Obesity is closely associated with pathological changes of the intestine including colon cancer, however little is known for possible molecular mechanisms. The objective of this study was to select candidate target genes which may be associated with abnormal cell growth and obesity‐related phynotipic biomarkers in the colon of mice fed high fat diet. C57BL/6J male mice were fed normal (ND) or high‐fat diet (HFD) over 8 weeks and sacrificed. Gene expressions of colon tissue were determined and those showing over 2‐fold changes in their signal intensity were analyzed for their correlations with obesity‐associated phenotypes including circulating adipokines. Mice fed with HFD exhibited significantly increased plasma leptin level compared with mice fed with ND. Expressions of Ces1 (carboxylesterase 1), Aldh1a1 (aldehyde dehydrogenase family 1) were down‐regulated while that of Gm5222 was up‐regulated in the colon tissue of HFD fed mice. The circulating leptin was negatively correlated with the expression of Aldh1a1 and Ces1. Aldh1a1 has been reported as a gene coding for a cytosolic enzyme involved in cell differentiation process, and therefore the down‐regulation of this gene may play a role in abnormal cell growth. [This research was supported by the SRC program (Center for Food & Nutritional Genomics: 2011‐0000912) of the NRF of Korea funded by the Ministry of Education, Science and Technology.]