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Anti‐obesity and inflammatory effects of Lactobacillus sp. OPK‐3 isolated from kimchi in cell line and in animal model
Author(s) -
Park Jeong-Eun,
Moon Yeon-Jeong,
Oh Suk-Heung,
Cha Youn-Soo
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.824.11
Subject(s) - biology , lipid metabolism , lactobacillus , proinflammatory cytokine , microbiology and biotechnology , lactobacillus casei , gene expression , inflammation , biochemistry , gene , immunology , fermentation
This study investigated the inhibitory effect of Lactobacillus sp.OPK‐3 isolated from kimchi ( L. sp.OPK‐3) on obesity and inflammatory cytokines using in vitro and in vivo animal model. 3T3‐L1 cells were treated with L. sp.OPK‐3 significantly reduced lipid accumulation. The mRNA levels of adipocyte‐specific marker genes were modulated by L. sp.OPK‐3. In vivo, five groups of C57BL/6J mice fed with a ND(Normal diet control), HFD(High fat diet control), HFD+ornithine, HFD+ L.brevis , and HFD+ L.sp. OPK‐3. All lactic acid bacteria supplemented groups had lower body weights and, more specifically, resulted in the Lactobacillus sp.OPK‐3 supplement group. Lipid profiles and inflammatory cytokines were improved by Lactobacillus sp. OPK‐3 when compared with HFD, ornithine and L.brevis groups. This study on regulation of gene expression which is related with inflammation, lipid and glucose metabolism, demonstrated the inter‐regulatory mechanism between transcription factor and sub‐gene, based on the gene expression change profiles by cDNA microarray. These results confirmed mRNA expression of enzymes related to lipid metabolism and inflammatory cytokines can be affected by the lactic acid bacteria. The results indicated that lactic acid bacteria from kimchi improve obesity and inflammatory cytokines through acting as a modulator of lipid metabolism (This work was supported by the National Research Foundation of Korea Grant funded by the Korean Government(MEST) (NRF‐2008‐331‐ E00371).