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Suppressive effect of Petasites japonicas extract on TPA‐induced ear edema in mouse model
Author(s) -
Seo Eun Young,
Kim Hyunae,
Kim Mee Ree
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.823.39
Subject(s) - medicine , tumor necrosis factor alpha , edema , proinflammatory cytokine , pharmacology , inflammation , anti inflammatory , herb , traditional medicine , medicinal herbs
Inflammation is a beneficial host response to foreign pathogens or tissue injury, and it eventually leads to the restoration of normal tissue structure and function. Petasites japonicas (Pj) is known as an herb for treating asthma, oxidant stress and gastric ulcer in traditional Oriental medicine. In this study, we evaluated the anti‐inflammatory effects of Pj extract on 12‐ O ‐tetradecanoylphorbol 13‐acetate (TPA)‐induced ear edema in mouse model. The Pj extract (10, 30, 50 mg) was acquired from whole plants of Petasites japonicas using butanol. After the PJ extract was administered to mice via gavage consecutively for 5 days, an edema was induced on the ear by topical application of 2.5 μg/ear of TPA dissolved in 10 μl of acetone. Ear thickness was measured using a Digimatic Micrometer (Mitsutoyo Co., Tokyo, Japan) before TPA application and 4 h after. ELISA was performed to determine the expression levels of cytokines. The levels of ear thickness were not significant between normal group and experimental groups, However, the ear weight in group Pj 10 was lower than that in TPA‐only group, and similar to that in normal group. TPA increased the expression of pro‐ inflammatory cytokines. The Pj extract significantly inhibited the release of interleukin (IL)‐ 1β, IL‐6 and tumor necrosis factor (TNF)‐α, when compared to TPA only. Especially, Pj at relatively low doses, reduced the level of pro‐inflammatory cytokines dose‐dependently. From present results, it was suggested that the extract of Petasites japonicas showed anti‐inflammatory action in TPA‐ induced ear edema mouse model.