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Examining the Anti‐inflammatory Properties of Blueberry Polyphenols Using MC3T3‐E1 Cells
Author(s) -
Elam Marcus L,
Zhang Jiyao,
Feresin Rafaela,
Hooshmand Shirin,
Arjmandi Bahram H
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.823.38
Subject(s) - rankl , runx2 , osteoblast , chemistry , tumor necrosis factor alpha , downregulation and upregulation , in vivo , polyphenol , gene expression , progenitor cell , microbiology and biotechnology , bone remodeling , in vitro , biochemistry , endocrinology , gene , stem cell , biology , activator (genetics) , antioxidant
The objective of this study was to investigate the mechanisms by which total blueberry polyphenol extract (BPE) exerts its anti‐inflammatory function on MC3T3‐E1 preosteoblasts under inflammatory conditions. Cells were treated with BPE at concentrations of 0, 10, 100 μg/ml. After 24 h incubation, cells were stimulated with TNF‐α (10 ng/ml) and IL‐1 (10 ng/ml) individually or in combination for 24 and 48 hrs. Cells and supernatants were collected to assess NO production and for RNA extraction. Select gene expressions involved in bone cell differentiation were assessed using commercial cDNA array plates. BPE was able to suppress the increased NO production induced by either TNF‐α or IL‐1, suggesting the prevention of impaired osteoblastic differentiation. However, BPE had no significant effect on NO production when TNF‐α and IL‐1 were both present. At the molecular level, BPE upregulated RANKL and IL‐6 via the NF‐κB signaling pathway. In spite of this, BPE tended to increase gene expression of Runx2 and osterix, albeit not significantly. From these preliminary findings it can be suggested that BPE does not suppress bone turnover through osteoblast‐mediated activity unlike in vivo findings. Grant Funding Source : Margaret A. Sittion Professorship