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Broccoli seedlings prevent glucose‐induced inflammation of peripheral blood mononuclear cells in humans
Author(s) -
Meijer Kees,
Jong Ingrid,
Koehorst Martijn,
Priebe Marion,
Roelofsen Han,
Vonk Roel
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.823.37
Subject(s) - sulforaphane , peripheral blood mononuclear cell , chemistry , inflammation , postprandial , nf κb , food science , biochemistry , medicine , endocrinology , biology , apoptosis , insulin , in vitro
We studied the effect of anti‐inflammatory components in vegetables on postprandial inflammation using broccoli seedlings (Broccocress®, Koppert Cress BV, Monster, The Netherlands), which are rich in the isothiocyanate sulforaphane (SFN). In an NF‐κB reporter cell line, increasing concentrations of 2–15 μM SFN lead to inhibition of TNFα‐stimulated NF‐κB activation up to 98% (p<0.05). Five healthy subjects ingested a load of 75 gram glucose with or without a smoothie of broccoli seedlings (45 min. prior to glucose). NF‐κB activation in the nuclear fraction of peripheral blood mononuclear cells was determined by ELISA. 75 gram glucose leads to a 36.5% ± 20.3% increase in NF‐κB activation. In combination with the smoothie, NF‐κB was decreased by 64.8% (p<0.005) and 29.3% ± 9.3% (p<0.005) compared to high‐glucose‐induced and basal NF‐κB levels, respectively. The level of NF‐κB activation correlated to blood glucose levels after the oral glucose load, but not when the smoothie was ingested as well. SFN could be detected in plasma after 1.5 hrs. Total SFN (free SFN plus conjugated SFN) correlated to inhibition of NF‐κB. This suggests that broccoli seedlings are effective in reducing metabolic inflammation in humans. Grant Funding Source : Food Nutrition Delta project # FND09002