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Anti‐inflammatory action of Liriope platyphylla (LP) on LPS‐stimulated RAW 264.7 macrophage
Author(s) -
Kim NaYeon,
Kim Min Jee,
Kim Mee Ree
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.823.35
Subject(s) - inflammation , lipopolysaccharide , tumor necrosis factor alpha , macrophage , nitric oxide , pharmacology , mapk/erk pathway , medicine , chemistry , immunology , kinase , biochemistry , in vitro
Liriope platyphylla (LP) is one of the well‐known herb use in eriental medicine for treatment asthma and bronchial and lung inflammation. In this study, we investigated the anti‐inflammatory effect of water extract of Liriope platyphylla (LP) on lipopolysaccharide (LPS)‐induced inflammation using RAW 264.7 macrophage cells. And for induction of inflammation in macrophages, RAW 264.7 cells (1×104) were stimulated with 3 ug/ml of LPS for 24 hr. Treatment of LP was carried out 15 hr before LPS stimulation in splenic RAW 264.7 cells. Anti‐inflammatory effect of LP on LPS‐induces inflammation in RAW 264.7 cells was measured the level of pro‐inflammatory cytokines (IL‐6 and TNF‐α), nitric oxide (NO) and PGE 2 produced from LPS‐stimulated RAW 264.7 cells. Treatment of LP suppressed the production of NO and pro‐inflammatory cytokines such as IL‐6 and TNF‐α from LPS‐treated RAW 264.7 cells. In addition, treatment with LP showed a significant decrease of PGE 2 , another inflammation parameter. In RT‐PCR analysis, it was ascertained that LP down‐regulated the expression of mRNA for iNOS, IL‐6 and TNF‐α in LPS‐stimulated RAW 264.7 cells. Furthermore, LP inhibited the NF‐κB activation induced by LPS, which was associated with the abrogation of IκB degradation. Moreover, treatment of LP suppressed the phosphorylation of ERK. JNK and p38 MAP kinases in LPS‐stimulated RAW 264.7 cell. It was of significant that administration of LP suppressed LPS‐induced endotoxemia in an animal model using mice. These results show that LP is able to suppress the production of IL‐4 and IFN‐γ inhibit LPS‐induced inflammation of RAW 264.7 macrophages. The anti‐inflammatory effect of LP on RAW 264.7 cells might be attributed to the inhibition of the expression of NO and pro‐inflammatory cytokines through the down‐regulation of NF‐κB activation and MAP kinase phosphorylation in macrophages. These findings suggest a possibility that LP is a good candidate available for endotoxin‐induced inflammation by macrophages.