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Polyphenols suppress oxidative stress in bovine articular chondrocytes
Author(s) -
Yagi Haruyo,
Ulici Veronica,
Tuan Rocky S
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.823.19
Subject(s) - oxidative stress , reactive oxygen species , polyphenol , chemistry , curcumin , nitric oxide , resveratrol , chondrocyte , biochemistry , pharmacology , antioxidant , biology , in vitro , organic chemistry
Reactive oxygen species (ROS) and nitric oxide (NO) have been implicated in chondrocyte senescence and cartilage aging, pathogenesis of osteoarthritis (OA) and rheumatoid arthritis. Naturally occurring polyphenolic compounds (polyphenols), such as curcumin (turmeric), resveratrol (grape), and epigallocatechin‐3‐gallate (EGCG) (green tea) have been known for their anti‐inflammatory and chondroprotective effects. However, the potential protective effects of these polyphenols against oxidative stress in chondrocytes are unclear. To investigate this, bovine articular chondrocytes were pre‐treated with polyphenols at varying concentrations, and then exposed to hydrogen peroxide (H 2 O 2 ) as an ROS inducer or S‐nitroso‐N‐acetylpenicillamine (SNAP) as a nitric oxide (NO) donor. Alternatively, chondrocytes were co‐treated with polyphenols and H 2 O 2 . Intracellular ROS/NO were measured using a fluorescent dye technique (H 2 DCFDA for ROS; DAF‐FM for NO). Direct interaction between polyphenols and H 2 O 2 was analyzed by quantitative peroxide assay. Our results showed that polyphenol pre‐treatment inhibited both H 2 O 2 ‐induced ROS and SNAP‐induced NO in a dose dependent‐manner in chondrocytes. Polyphenol co‐treatment also inhibited both ROS and NO production in chondrocytes. Interestingly, our results indicate that all three polyphenols rapidly and completely blocked H 2 O 2 ‐induced ROS without significant direct effect on H 2 O 2 itself. Further, we found that only EGCG rapidly suppressed inducible NO production. These findings demonstrate that polyphenols are capable of suppressing inducible oxidative stress in chondrocytes, and suggest their potential therapeutic value for clinical application. Support: PA Commonwealth Dept of Health