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In Vitro Evidence of Hepato‐ and Neuro‐Protective Effects of Fish Press Juices against Hydrogen Peroxide‐Induced Oxidative Stress
Author(s) -
Waly Mostafa,
Al-Rizeiqi Mohammed,
Al-Sabahi Jamal,
Soussi Bassam
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.823.13
Subject(s) - oxidative stress , antioxidant , glutathione , reactive oxygen species , hydrogen peroxide , chemistry , oxidative phosphorylation , biochemistry , enzyme
Background Oxidative stress induced by free radicals and reactive oxygen species, depletes intracellular glutathione (GSH) and inhibits antioxidant enzymes. Consumption of antioxidant‐rich foods protects against oxidative stress‐related health problems. Objective This study aimed to (1) measure the antioxidants contents of the press juices (PJs) extracted from Hamour and King Fish and (2) Evaluate the PJs effect on oxidative stress induced by hydrogen peroxide (H2O2), using human cultured neuronal cells (SH‐SY5Y) and hepatic cells (HepG2). Results PJs analysis revealed the presence of significant amounts of antioxidants, vitamin C and GSH in both fish species. The total antioxidant capacity (TAC) of Hamour PJ was significantly higher than that of Kingfish PJ. H2O2‐induced oxidative stress in SH‐SY5Y and HepG2 as presented by GSH depletion and antioxidant enzymes inhibition. PJs protect against the H2O2‐induced cellular oxidative stress. Conclusion PJs from Hamour and Kingfish, locally available fishes in Oman, are powerful dietary sources for antioxidants and protect against oxidative stress. Further experimental animals studies are required to further investigate the in vivo effect of PJs against oxidative stress and its related health problems.