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Effect of dietary fat on carbohydrate response element binding protein in a rat model of fatty liver and insulin resistance
Author(s) -
Kuo Yu-Fu,
Harper Marianna A.,
Clarke Steven,
Axen Kenneth,
Axen Kathleen
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.819.43
Subject(s) - carbohydrate responsive element binding protein , medicine , basal (medicine) , endocrinology , insulin resistance , carbohydrate , chemistry , fatty liver , insulin , biology , biochemistry , transcription factor , disease , gene
The effect of obesity and insulin resistance on content and translocation of carbohydrate response element binding protein (ChREBP) was studied in dietary obese rats. Method Male Sprague‐Dawley rats were separated into: LF (N=12) fed 55% carbohydrate: 15% fat (% kcal) and HF (N=8) fed 19% carbohydrate: 55% fat ad libitum for 8 weeks. Liver samples were collected from LF and HF after 20 h food deprivation (D) and then 3 h after oral glucose load (G). Liver protein extracts and homogenates, separated into cytoplasmic and nuclear fractions were analyzed by immunoblotting. Results In the deprived state, total ChREBP was greater in HF than LF rats (P<0.05). However, after the glucose load the level of total ChREBP was increased in LF, but not HF rats, resulting in similar levels of total ChREBP in the two groups. In addition, both groups showed similar increases in nuclear ChREBP content after the glucose load. Conclusion Higher basal levels of ChREBP protein in the obese group suggest higher basal hepatic fat synthesis than in the lean group. Given that post‐glucose ChREBP protein content and nuclear translocation were similar in both groups, the increase in ChREBP‐stimulated fat synthesis by glucose may be greater in the LF vs. HF group. Grant number:1 SC3 GM086298 ‐01A1 Grant Funding Source : SC3GM086298