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Effects of Mungbean extract and Mungbean tesa extract on adipogenesis and obesity‐related inflammation in vitro 3T3L1 cells and in vivo KK‐Ay mice
Author(s) -
Kang Inhae,
Chang E,
Yang SJ,
Quan ZJ,
Park MY,
Choi MJ,
Kim JI,
Wi HR,
Choi SL,
Lee M
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.818.8
Subject(s) - adipogenesis , adipocyte , in vivo , endocrinology , medicine , ampk , metformin , inflammation , lipid metabolism , glycemic , chemistry , pharmacology , diabetes mellitus , adipose tissue , biology , biochemistry , enzyme , microbiology and biotechnology , protein kinase a
Obesity is associated with increased metabolic diseases such as hyperlipidemia, type2 DM. The Mungbean(MB) is known as lower Glycemic Index containing food thereby decrease lipid profiles. The purpose of this study is to investigate the inhibitory effects of MB on adipocyte differentiation and insulin sensitivity. The isolated component from MB was treated in dose dependent in 3T3‐L1. Treatment was for 7 days from differentiation induction to fully adipocyte differentiation. Compared to vehicle, lipid accumulation was significantly lower in dose dependent manner. To investigate anti‐obesity, anti‐diabetic effects of MB, MB testa in vivo, we used type2 diabetic model, KK‐Ay mice. MB extract(MBE), MB testa extract(MBT) were orally administrated for 4wks with 60% highfat diet. First of all, AST, ALT were not different compared to the control. Two extract of MBE, MBT significantly lowered IL‐6 levels in plasma but lowered TNF‐a in muscle. Since PPAR¥ã, ACC, C/EBP¥á and AMPK gene expression was decreased by two extracts compared to control and Metformin treated group, MBE, MBT inhibited metabolism of adipogenesis in liver. Most important results was that MBE, MBT reduced epididymal fat weight and reduced those gene expression. In conclusion, MB exerts an anti‐obesity, anti‐diabetic effects. This work is supported by Cooperative Research Program for Agriculture Science&Technology Development(PJ907089) Grant Funding Source : Agriculture Science&Technology Development, Korea, Republic of