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Ceramide synthase 6 suppression leads to resistance to photodynamic therapy in human head and neck cancer cells
Author(s) -
Separovic Duska,
Breen Paul,
Joseph Nicholas,
Gudz Tatyana I.
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.798.28
Subject(s) - ceramide , apoptosis , gene knockdown , head and neck squamous cell carcinoma , sphingolipid , cancer research , ceramide synthase , downregulation and upregulation , cancer cell , photodynamic therapy , cancer , head and neck cancer , chemistry , biology , medicine , microbiology and biotechnology , biochemistry , organic chemistry , gene
Defective apoptosis contributes to resistance to cancer treatment by photodynamic therapy (PDT). Ceramide synthase 6 (CerS6) has been implicated in apoptosis. Here we tested the role of CerS6 downregulation in apoptosis after PDT with the silicone phthalocyanine Pc 4 in human head and neck squamous carcinoma cells. CerS6 knockdown led to reduction in PDT‐induced DEVDase activation, mitochondrial depolarization, apoptosis, and cell killing. CerS6 knockdown also resulted in distinct changes in sphingolipid profile after PDT. Overall, our data show a novel role for CerS6 in regulating resistance to PDT.