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Attachment capabilities of erythroleukemia cells to normal human fibroblasts
Author(s) -
Barnes Carol,
Birckbichler Paul J
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.798.18
Subject(s) - k562 cells , cell culture , extracellular matrix , cell , microbiology and biotechnology , chemistry , extracellular , biochemistry , biology , genetics
Erythroleukemia cell lines, K562 and HEL, show a differential response to tissue transglutaminase (tTg) activation following exposure to retinoic acid. To study a role for tTg in cell‐¬cell attachment both cell lines were independently added to preconfluent and confluent normal human fibroblasts (WI‐38). No cell attachment was evident 6 hours after 175,000 K562 cells were added to adherent WI‐38 cells. After 12 hours small numbers of single cells adhered to the WI‐38 cells. Cell attachment continued through 48 hours with colonies evident as large as 3 to 5 cells with the greatest attachment occurring in the confluent cultures. To monitor cell growth during the 48 hour period, a control of K562 cells identical to the experimental flask was set up. The suspended cells in the experimental cultures were routinely 80 to 90% of those in the control K562 culture, indicating 10 to 20% of the K562 cells at any given time were attached to the WI‐38 cells. The attachment was sufficiently strong such that manual agitation failed to remove the attached cells. These observations suggest that a stabilized extracellular matrix played a role in the attachment. The failure to remove all the attached cells by agitation raises the possibility of a covalent linkage (bridge) between the WI‐38 and K562 cells; possibly a disulfide and/or isopeptide bond(s). Support from College of Health, Environment and Science and Slippery Rock University.