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Insecticide Toxicity and Characterization of Cytochrome P450 CYP6BB1 and CYP6P10 in Aedes sollicitans Larvae
Author(s) -
Suwanchaichinda Chansak,
Brattsten Lena B
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.784.3
Subject(s) - biology , carbaryl , larva , cytochrome p450 , population , monooxygenase , toxicology , vector (molecular biology) , spinosad , toxicity , gene , aedes , insecticide resistance , pesticide , zoology , ecology , genetics , enzyme , biochemistry , chemistry , demography , organic chemistry , sociology , recombinant dna
Cytochrome P450 monooxygenases (P450s) are involved in biotransformation of endogenous and exogenous compounds, including insecticides, which often result in insecticide resistance in insects. Two P450‐encoding genes, CYP6BB1 and CYP6P10, were identified in Aedes sollicitans , an Eastern Equine Encephalitis vector. We examined mosquitoes collected in Atlantic County, New Jersey, where multiple insecticides have been used. We found that pre‐exposure of the mosquito larvae to pentamethylbenzene (PMB) significantly decreased the toxicity of carbaryl, but not that of spinosad. Gene expression of CYP6BB1 was slightly up‐regulated upon induction by PMB, whereas CYP6P10 remained unchanged. We also compared the Atlantic mosquitoes to another population collected from a location on Long Island where insecticides have not been used. We found that the gene expression level of CYP6P10 in the Long Island population was considerably higher than that in the Atlantic population. The gene expression level of CYP6BB1 was also higher in the Long Island mosquitoes. We concluded that PMB induced P450s that detoxified carbaryl. Whether these two genes are involved in the insecticide detoxification in the mosquitoes will require further studies. (Supported by Rutgers Hatch Fund)