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Modulation of H 2 O 2 production in vitro by low level magnetic fields
Author(s) -
Martino Carlos F,
Castello Pablo
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.783.3
Subject(s) - ht1080 , superoxide dismutase , chemistry , horseradish peroxidase , catalase , magnetic field , nuclear magnetic resonance , in vitro , physics , oxidative stress , biochemistry , enzyme , quantum mechanics
Background In this paper, we present data on the influence of low level magnetic fields (0.5 μT‐2 μT) on the modulation of hydrogen peroxide (H 2 O 2 ) biogenesis in human fibrosarcoma cancer cell line HT1080, and bovine pulmonary artery endothelial cells (PAEC) compared to cells exposed to geomagnetic field (control; 45 μT‐60 μT). Methods The extracellular quantification of the production of H 2 O 2 may be a direct measure of the production and decomposition of superoxide radical. H 2 O 2 can freely pass through cell membranes and can be detected outside the cell. For the quantification of H 2 O 2 we used a method that has been successfully applied before. This method involves the oxidation of Amplex Red by H 2 O 2 in the presence of horseradish peroxidase (HRP). Tri‐axial Helmholtz coils established the control static magnetic field. Low level fields were implemented by a μ–metal cylinder. The background time‐varying magnetic field was measured by induction with a sensor comprised of a set of square perpendicular coils. Results Reduction of the Earth's magnetic field suppressed H 2 O 2 production in cancer cells and PAEC. The addition of catalase and superoxide dismutase (SOD) mimetic MnTBAP inhibited the magnetic field effect on H 2 O 2 production. Conclusion Because most cancer cells undergo increased oxidative stress and ROS production due to excessive metabolic requirements to support an increased rate of cell division, it is reasonable to consider investigating a therapeutic that might target cancer cells through a free radical mechanism. Whether radio frequency exposure can influence growth rates and H 2 O 2 production remains open and we propose to investigate it in the future. Modulating ROS production by magnetic fields may open new venues of biomedical research and therapeutic strategies.