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Proteomic evaluation of a caveolae‐enriched fraction from variable beta3 integrin backgrounds
Author(s) -
Liu Jinsha,
Jin Lianji,
Bush Jason A
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.776.12
Subject(s) - integrin , microbiology and biotechnology , biology , caveolin , endocytosis , caveolae , endosome , cell , cancer research , signal transduction , biochemistry , intracellular
Both integrins and caveolins are known tumor biomarkers for early and late‐stage cancers. Caveolin‐1 can function either as a tumor suppressor or promoter depending on tumor origin. Excess integrins are generally associated with cancer progression/angiogenesis but some integrin knockout mouse models have increased tumor burden. This dichotomy is compounded by data indicating that specific integrin subunits can bind caveolins. We hypothesize that the mechanism of integrin endocytosis/recycling is dictated by the combination of integrin subunits present on the cell surface and the interacting caveolin members. Our project focuses on cellular systems where the beta3 integrin subunit is overexpressed having a profound effect on cell functions. Using a combination of RT‐PCR, subcellular fractionation, immunoblotting, and proteomic workflows, our preliminary data indicate that 1) expression of caveolin isoforms changes in variable beta3 integrin backgrounds, 2) the contingent of proteins from the endosomal compartment changes with respect to beta3 integrin expression levels. Taken together, this data provides further understanding for molecular interactions associated with integrin recycling and has implication for cancer cell motility.