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High throughput transcriptomic analysis of the effects of radiation exposure in a mouse model
Author(s) -
Miller Stacy-Ann,
Shupp Jeffrey W,
Moffatt Lauren T,
Rosenthal Dean S,
Nam Jason,
Hammamieh Rasha,
Jett Marti
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.774.1
Subject(s) - biodosimetry , dna damage , dna repair , biology , transcriptome , gene , gene expression , histone , dna microarray , microarray , microbiology and biotechnology , dna , cancer research , genetics , ionizing radiation , irradiation , physics , nuclear physics
Early detection of radiation exposure is important in the treatment of radiation injury and appropriate triage of patients. Detecting the cellular response to DNA double‐strand breaks (DSBs) has been thought to be a valid form of biodosimetry. A target biomarker for this is the rapid phosphorylation of H2AX, which is the minor histone H2A variant at serine residue‐139 to produce γ‐H2AX. Although the use of immunofluorescence‐based assays to visualize discrete γ‐H2AX foci has resulted in a sensitive and effective method for detecting DSBs, more sensitive and easy to analyze biomarkers of radiation exposure are desired. Here, a series of C57Bl6 mice were exposed to varying doses of X‐ray radiation and skin biopsies were harvested over a 4‐week time course. Subsequent to nucleic acid isolation, cDNA microarrays were used to study differential gene expression. We found that genes involved in response to stress and cell adhesion are decreased, while genes involved in mitosis, coagulation, lipoprotein metabolism, and protease inhibitor activities were increased following exposure. Further work will be directed at understanding the dose‐response nature of these signature gene responses.