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The Box C/D sRNP dimeric architecture is conserved across Kingdom Archaea
Author(s) -
Baserga Susan J.,
Phipps Kathleen R.,
Taylor David W.,
Wang Hongwei
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.773.2
Subject(s) - sulfolobus solfataricus , archaea , ribonucleoprotein , biology , rna , genetics , transfer rna , gene
Site‐directed 2′‐ O‐ methylation of RNA is catalyzed, in eukaryotes and archaea, by box C/D small (nucleolar) ribonucleoproteins (box C/D s(no) RNPs). Recent structural studies have led to a controversy over whether box C/D sRNPs are monomeric or dimeric macromolecules. Here we examine the role of the omnipresent sRNA loop as a structural determinant of multimeric sRNP assembly. Mutational analysis of the sRNA shows that the conserved loop between boxes C′ and D′ is required for dimeric sRNP formation, and explains how mutations in sRNAs can effect oligomeric architecture. In this study, we present negative‐stain electron microscopy single particle reconstructions of catalytically active dimeric box C/D sRNPs from three distantly related archaeal species, Solfolobus solfataricus, Pyrococcus abyssi, and Archaeoglobus fulgidus, Our results demonstrate that the dimeric architecture of box C/D sRNPs is broadly conserved across the Archaeal Kingdom.